You'll need to put a ground plane on the fiberglass roof and then do some measurements and tuning.
Without getting really complicated (which I'm not sure I can effectively do any longer), some antennas are designed to match the transmitter without a ground plane (or counterpoise) - the link in my earlier post featured antennas designed for mounting on fiberglass with no metal substrate. At CB frequencies (27 Mhz), ideal antennas are a bit long for mobile applications, so all of them are a less than ideal compromise.
As taoshum suggests, if the antenna can be adjusted (or tuned), it would be a good idea to do that if you have the capability.